Recombinant humaan PP2C gamma/PPM1G eiwit

PPM1G, as known as protein phosphatase 1G, is a member of the PP2C family of Ser/Thr protein phosphatases that is negative regulators of cell stress response pathways. This enzyme localizes to the cytoplasm and is widely expressed, with most abundant expression detected in testis, skeletal muscle and heart. It is necessary for the dephosphorylation of pre-mRNA splicing factors, which is an important process for the formation of the functional spliceosome. Recombinant human PPM1G protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 85% by SDS-PAGE

61.4 kDa (566aa)

Protein phosphatase, Mg2+/Mn2+ dependent 1G, Protein phosphatase Mg2+/Mn2+ dependent 1G, Protein phosphatase magnesium-dependent 1 gamma, Protein phosphatase magnesium dependent 1 gamma, Protein phosphatase 2C gamma isoform, Protein phosphatase 2 catalytic subunit gamma isoform, Protein phosphatase 1G, formerly 2C, magnesium-dependent, gamma isoform, Protein phosphatase 1G, Protein phosphatase 1C, PPP2CG, PPM1G, PPM1C, PP2Cgamma, PP2CG, PP2C gamma, PP2C g, PP2C, MGC2870, MGC1675, ATGP3784-10 µg, ATGP3784-20 µg, ATGP3784-50 µg, ATGP3784-100 µg, ATGP3784-250 µg, ATGP3784-500 µg, ATGP3784-1 mg, ATGP3784-010, ATGP3784-020, ATGP3784-050, ATGP3784-100, ATGP3784-250, ATGP3784-500, ATGP3784-01M

Travis SM., et al. (1997) FEBS Lett. 412 (3) :415-9.; ; Brautigan D.L., et al. (2005) Biochemistry. 44 (33) :11067-73.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl , 0.1mM PMSF

Cell Cycle

NP_817092

O15355

Human

MGAYLSQPNT VKCSGDGVGA PRLPLPYGFS AMQGWRVSME DAHNCIPELD SETAMFSVYD GHGGEEVALY CAKYLPDIIK DQKAYKEGKL QKALEDAFLA IDAKLTTEEV IKELAQIAGR PTEDEDEKEK VADEDDVDNE EAALLHEEAT MTIEELLTRY GQNCHKGPPH SKSGGGTGEE PGSQGLNGEA GPEDSTRETP SQENGPTAKA YTGFSSNSER GTEAGQVGEP GIPTGEAGPS CSSASDKLPR VAKSKFFEDS EDESDEAEEE EEDSEECSEE EDGYSSEEAE NEEDEDDTEE AEEDDEEEEE EMMVPGMEGK EEPGSDSGTT AVVALIRGKQ LIVANAGDSR CVVSEAGKAL DMSYDHKPED EVELARIKNA GGKVTMDGRV NGGLNLSRAI GDHFYKRNKN LPPEEQMISA LPDIKVLTLT DDHEFMVIAC DGIWNVMSSQ EVVDFIQSKI SQRDENGELR LLSSIVEELL DQCLAPDTSG DGTGCDNMTC IIICFKPRNT AELQPESGKR KLEEVLSTEG AEENGNSDKK KKAKRD