Recombinant humaan PPA2-eiwit

PPA2 is localized to the mitochondrion, is highly similar to members of the inorganic pyrophosphatase (PPase) family, and contains the signature sequence essential for the catalytic activity of PPase. PPases catalyze the hydrolysis of pyrophosphate to inorganic phosphate, which is important for the phosphate metabolism of cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. Recombinant human PPA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Inorganic pyrophosphatase 2 mitochondrial isoform 1, Inorganic pyrophosphatase 2, mitochondrial isoform 1, Pyrophosphatase (inorganic) 2, HSPC124, SID6-306

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

37.1 kDa (325aa) confirmed by MALDI-TOF

PPA2, Inorganic pyrophosphatase 2 mitochondrial isoform 1, Inorganic pyrophosphatase 2, mitochondrial isoform 1, Pyrophosphatase (inorganic) 2, HSPC124, SID6-306, ATGP2771-10 µg, ATGP2771-20 µg, ATGP2771-50 µg, ATGP2771-100 µg, ATGP2771-250 µg, ATGP2771-500 µg, ATGP2771-1 mg, ATGP2771-10, ATGP2771-20, ATGP2771-50, ATGP2771-100, ATGP2771-250, ATGP2771-500, ATGP2771-1

Gauthier-Campbell C., Bredt D.S., et al. (2004) Mol. Biol. Cell 15:2205-2217

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_789845

Q9H2U2

Human

MGSSHHHHHH SSGLVPRGSH MGSALYHTEE RGQPCSQNYR LFFKNVTGHY ISPFHDIPLK VNSKEENGIP MKKARNDEYE NLFNMIVEIP RWTNAKMEIA TKEPMNPIKQ YVKDGKLRYV ANIFPYKGYI WNYGTLPQTW EDPHEKDKST NCFGDNDPID VCEIGSKILS CGEVIHVKIL GILALIDEGE TDWKLIAINA NDPEASKFHD IDDVKKFKPG YLEATLNWFR LYKVPDGKPE NQFAFNGEFK NKAFALEVIK STHQCWKALL MKKCNGGAIN CTNVQISDSP FRCTQEEARS LVESVSSSPN KESNEEEQVW HFLGK