Recombinant humaan PPCDC-eiwit

PPCDC belongs to the HFCD (homo oligomeric flavin containing Cys decarboxylase) superfamily that plays a role in the biosynthesis of coenzyme A (CoA) from pantothenate (Vitamin B) . The biosynthesis of CoA from pantothenate requires several steps: the phosphorylation of pantothenate, the conversion of 4'-hosphopantothenate to 4'-phosphopantetheine, the adenylation by phosphopantetheine adenylyltransferase to form dephospho-CoA and the phosphorylation by dephospho-CoA kinase to form CoA. PPCDC plays a direct role in this pathway by converting 4'-phosphopantothenate into 4'-phosphopantetheine. Recombinant human PPCDC protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.6 kDa (224aa) confirmed by MALDI-TOF

PPCDC, Phosphopantothenoylcysteine decarboxylase, MDS018, PPC-DC, COAC, FLJ14585, ATGP0748-10 µg, ATGP0748-20 µg, ATGP0748-50 µg, ATGP0748-100 µg, ATGP0748-250 µg, ATGP0748-500 µg, ATGP0748-1 mg, ATGP0748-10, ATGP0748-20, ATGP0748-50, ATGP0748-100, ATGP0748-250, ATGP0748-500, ATGP0748-1

Strauss E., et al. (2004) Biochemistry. 43 (49) :15520-33. ; ; Daugherty M., et al. (2002) J Biol Chem. 277 (24) :21431-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Metabolism

AAH14409

Q96CD2

Human

MGSSHHHHHH SSGLVPRGSH MEPKASCPAA APLMERKFHV LVGVTGSVAA LKLPLLVSKL LDIPGLEVAV VTTERAKHFY SPQDIPVTLY SDADEWEMWK SRSDPVLHID LRRWADLLLV APLDANTLGK VASGICDNLL TCVMRAWDRS KPLLFCPAMN TAMWEHPITA QQVDQLKAFG YVEIPCVAKK LVCGDEGLGA MAEVGTIVDK VKEVLFQHSG FQQS