Recombinant humaan PPCS-eiwit

PPCS, also known as phosphopantothenate--cysteine ligase, catalyzes the first step in the biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5), which is an essential universal pathway in prokaryotes and eukaryotes. PPCS, one of the last enzymes in this pathway, converts phosphopantothenate to phosphopantothenoylcysteine. Recombinant human PPCS protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Phosphopantothenate--cysteine ligase, COAB, PPC synthetase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

36.1 kDa (331aa) confirmed by MALDI-TOF

PPCS, Phosphopantothenate--cysteine ligase, COAB, PPC synthetase, ATGP1064-10 µg, ATGP1064-20 µg, ATGP1064-50 µg, ATGP1064-100 µg, ATGP1064-250 µg, ATGP1064-500 µg, ATGP1064-1 mg, ATGP1064-10, ATGP1064-20, ATGP1064-50, ATGP1064-100, ATGP1064-250, ATGP1064-500, ATGP1064-1

Manoj N. et al. (2003) Structure. 11 : 927-936.; ; Christina Spry. et al. (2009) J Biol Chem. 284: 24904-24913.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_078940

Q9HAB8

Human

MGSSHHHHHH SSGLVPRGSH MAEMDPVAEF PQPPGAARWA EVMARFAARL GAQGRRVVLV TSGGTKVPLE ARPVRFLDNF SSGRRGATSA EAFLAAGYGV LFLYRARSAF PYAHRFPPQT WLSALRPSGP ALSGLLSLEA EENALPGFAE ALRSYQEAAA AGTFLAVEFT TLADYLHLLQ AAAQALNPLG PSAMFYLAAA VSDFYVPVSE MPEHKIQSSG GPLQITMKMV PKLLSPLVKD WAPKAFIISF KLETDPAIVI NRARKALEIY QHQVVVANIL ESRQSFVFIV TKDSETKLLL SEEEIEKGVE IEEKIVDNLQ SRHTAFIGDR N