Recombinant humaan PPM1D-eiwit

PPM1D also known as Protein phosphatase 1D magnesium-dependent, delta isoform. This protein is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase (MAPK/p38) through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. Recombinant human PPM1D, fused to His-tag at N-terminus, was expressed in E. coli.

Protein phosphatase 1D magnesium-dependent delta isoform, Protein phosphatase 1D magnesium-dependent, delta isoform, PP2C-DELTA, WIP1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

33.2 kDa (299aa)

PPM1D, ATGP2992-10 µg, ATGP2992-20 µg, ATGP2992-50 µg, ATGP2992-100 µg, ATGP2992-250 µg, ATGP2992-500 µg, ATGP2992-1 mg, ATGP2992-10, ATGP2992-20, ATGP2992-50, ATGP2992-100, ATGP2992-250, ATGP2992-500, ATGP2992-1

Fiscella M., et al. (1997) Proc Natl Acad Sci u S A, 94 (12) : 6048-53.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Signal Transduction

AAH33893

Q8NEA7

Human

VAFFAVCDG HGGREAAQFA REHFWGFIKK QKGFTSSEPA KVCAAIRKGF LACHLAMWKK LAEWPKTMTG LPSTSGTTAS VVIIRGMKMY VAHVGDSGVV LGIQDDPKDD FVRAVEVTQD HKPELPKERE RIEGLGGSVM NKSGVNRVVW KRPRLTHNGP VRRSTVIDQI PFLAVARALG DLWSYDFFSG EFVVSPEPDT SVHTLDPQKH KYIILGSDGL WNMIPPQDAI SMCQDQEEKK YLMGEHGQSC AKMLVNRALG RWRQRMLRAD NTSAIVICI