Recombinant humaan PPME1-eiwit

PPME1, also known protein phosphatase methylesterase-1 (PME1), catalyzes the demethylation and inactivation of protein phosphatase (PP2A), which is a multimeric phosphoserine/ threonine protein phosphatase associated with growth inhibition and cell cycle arrest. It can demethylate PP2A catalytic subunit in vitro and okadaic acid treatment is capable of inhibiting this reaction. It is conserved from yeast to human and contains a motif found in lipases having a catalytic triad activated serine as their active site nucleophile. Recombinant human PPME1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Protein phosphatase methylesterase 1, FLJ22226, PME-1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

44.4 kDa (406aa) confirmed by MALDI-TOF

PPME1, Protein phosphatase methylesterase 1, FLJ22226, PME-1, ATGP1150-10 µg, ATGP1150-20 µg, ATGP1150-50 µg, ATGP1150-100 µg, ATGP1150-250 µg, ATGP1150-500 µg, ATGP1150-1 mg, ATGP1150-10, ATGP1150-20, ATGP1150-50, ATGP1150-100, ATGP1150-250, ATGP1150-500, ATGP1150-1

Ogris E. et al. (1999) J. Biol. Chem. 274: 14382-14391.; ; Gagnon S.N. et al. (2002) Biochem. J. 368:263-271.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Signal Transduction

NP_057231

Q9Y570

Human

MGSSHHHHHH SSGLVPRGSH MSALEKSMHL GRLPSRPPLP GSGGSQSGAK MRMGPGRKRD FSPVPWSQYF ESMEDVEVEN ETGKDTFRVY KSGSEGPVLL LLHGGGHSAL SWAVFTAAII SRVQCRIVAL DLRSHGETKV KNPEDLSAET MAKDVGNVVE AMYGDLPPPI MLIGHSMGGA IAVHTASSNL VPSLLGLCMI DVVEGTAMDA LNSMQNFLRG RPKTFKSLEN AIEWSVKSGQ IRNLESARVS MVGQVKQCEG ITSPEGSKSI VEGIIEEEEE DEEGSESISK RKKEDDMETK KDHPYTWRIE LAKTEKYWDG WFRGLSNLFL SCPIPKLLLL AGVDRLDKDL TIGQMQGKFQ MQVLPQCGHA VHEDAPDKVA EAVATFLIRH RFAEPIGGFQ CVFPGC