Recombinant humaan PQBP1-eiwit

Polyglutamine binding protein 1, also known as PQBP1, is a transcription repressor that associates with polyglutamine tract-containing transcription regulators and causative genes for neurodegenerative disorders. PQBP-1 localizes to the nucleus and is present in neurons throughout the brain, with abundant levels in hippocampus, cerebellar cortex and olfactory bulb. PQBP-1 contains a WWP/WW domain that binds proline-rich motifs and a C2 domain that can influence Ca2+-dependent phospholipid signaling. Recombinant human PQBP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Polyglutamine binding protein 1, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33 kDa (289aa) confirmed by MALDI-TOF

PQBP1, Polyglutamine binding protein 1, MRX55, MRXS3, MRXS8, NPW38, RENS1, SHS, ATGP1540-10 µg, ATGP1540-20 µg, ATGP1540-50 µg, ATGP1540-100 µg, ATGP1540-250 µg, ATGP1540-500 µg, ATGP1540-1 mg, ATGP1540-10, ATGP1540-20, ATGP1540-50, ATGP1540-100, ATGP1540-250, ATGP1540-500, ATGP1540-1

Waragai M., et al. (1999) Hum Mol Genet. 8:977-987.; ; Waragai M C., et al. (2000) Biochem Biophys Res. 273: 592-595.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_005701.1

O60828

Human

MPLPVA LQTRLAKRGI LKHLEPEPEE EIIAEDYDDD PVDYEATRLE GLPPSWYKVF DPSCGLPYYW NADTDLVSWL SPHDPNSVVT KSAKKLRSSN ADAEEKLDRS HDKSDRGHDK SDRSHEKLDR GHDKSDRGHD KSDRDRERGY DKVDRERERD RERDRDRGYD KADREEGKER RHHRREELAP YPKSKKAVSR KDEELDPMDP SSYSDAPRGT WSTGLPKRNE AKTGADTTAA GPLFQQRPYP SPGAVLRANA EASRTKQQD