Recombinant humaan PRKAR2A-eiwit

PRKAR2A also known as cAMP-dependent protein kinase type II-alpha regulatory subunit. This subunit can be phosphorylated by the activated catalytic subunit. It may interact with various A-kinase anchoring proteins and determine the subcellular localization of cAMP-dependent protein kinase. Recombinant human PRKAR2A, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

48.6 kDa (434aa) confirmed by MALDI-TOF

PRKAR2A, cAMP-dependent protein kinase type II-alpha regulatory subunit, PKR2, PRKAR2, ATGP3050-10 µg, ATGP3050-20 µg, ATGP3050-50 µg, ATGP3050-100 µg, ATGP3050-250 µg, ATGP3050-500 µg, ATGP3050-1 mg, ATGP3050-10, ATGP3050-20, ATGP3050-50, ATGP3050-100, ATGP3050-250, ATGP3050-500, ATGP3050-1

Yang H., et al. (2013) J. Biol. Chem. 288 (12), 8737-8749

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol, 1mM DTT

Signal Transduction

NP_004148

P13861

Human

MGSSHHHHHH SSGLVPRGSH MGSMSHIQIP PGLTELLQGY TVEVLRQQPP DLVEFAVEYF TRLREARAPA SVLPAATPRQ SLGHPPPEPG PDRVADAKGD SESEEDEDLE VPVPSRFNRR VSVCAETYNP DEEEEDTDPR VIHPKTDEQR CRLQEACKDI LLFKNLDQEQ LSQVLDAMFE RIVKADEHVI DQGDDGDNFY VIERGTYDIL VTKDNQTRSV GQYDNRGSFG ELALMYNTPR AATIVATSEG SLWGLDRVTF RRIIVKNNAK KRKMFESFIE SVPLLKSLEV SERMKIVDVI GEKIYKDGER IITQGEKADS FYIIESGEVS ILIRSRTKSN KDGGNQEVEI ARCHKGQYFG ELALVTNKPR AASAYAVGDV KCLVMDVQAF ERLLGPCMDI MKRNISHYEE QLVKMFGSSV DLGNLGQ