Recombinant humaan PRL-3/PTP4A3-eiwit

Protein tyrosine phosphatase type IVA 3 (PRL-3), as known as PTP4A3, belongs to a small class of prenylated protein tyrosine phosphatases (PTPs) that remove phosphate modifications from tyrosine residues on proteins. This protein enhances cell proliferation, cell motility and invasive activity. High levels of PRL-3 expression are associated with tumorigenesis and metastasis, thus it is overexpressed in metastatic colorectal, ovarian, liver and skin cancers. Recombinant human PRL-3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Protein tyrosine phosphatase type IVA 3, PTP4A3, PRL3, Protein tyrosine phosphatase type IVA 3 Potentially prenylated protein tyrosine phosphatase, PRL 3, PRL R, PRLR, Protein tyrosine phosphatase 4a3, PTP 4A3, Protein Tyrosine Phosphatase Type IVA Member 3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

21.6 kDa (193aa) confirmed by MALDI-TOF

PRL3, PTP4A3, Protein tyrosine phosphatase type IVA 3, PTP4A3, PRL3, Protein tyrosine phosphatase type IVA 3 Potentially prenylated protein tyrosine phosphatase, PRL 3, PRL R, PRLR, Protein tyrosine phosphatase 4a3, PTP 4A3, Protein Tyrosine Phosphatase Type IVA Member 3, ATGP0398-10 µg, ATGP0398-20 µg, ATGP0398-50 µg, ATGP0398-100 µg, ATGP0398-250 µg, ATGP0398-500 µg, ATGP0398-1 mg, ATGP0398-10, ATGP0398-20, ATGP0398-50, ATGP0398-100, ATGP0398-250, ATGP0398-500, ATGP0398-1

Ooki A., et al. (2009) . Oncol Rep. 21 (6) :1467-75; ; Wang Z., et al. (2009) . Ann Surg Oncol. 16 (1) :208-19.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM EDTA, 1mM DTT, 10% glycerol

Enzymes & Proteases

NP_116000

O75365

Human

MGSSHHHHHH SSGLVPRGSH MARMNRPAPV EVSYKHMRFL ITHNPTNATL STFIEDLKKY GATTVVRVCE VTYDKTPLEK DGITVVDWPF DDGAPPPGKV VEDWLSLVKA KFCEAPGSCV AVHCVAGLGR APVLVALALI ESGMKYEDAI QFIRQKRRGA INSKQLTYLE KYRPKQRLRF KDPHTHKTRC CVM