Recombinant humaan PRMT3-eiwit

PRMT3, as known as protein arginine N-methyltransferase 3, belongs to the protein arginine methyltransferase family. This enzyme catalyzes the methylation of guanidino nitrogens of arginyl residues of proteins. It acts on 40S ribosomal protein S2 (rpS2), which is its major in-vivo substrate, and is involved in the proper maturation of the 80S ribosome. Recombinant human PRMT3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein arginine N-methyltransferase 3 isoform 1, HRMT1L3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

62.3 kDa (554aa)

PRMT3, Protein arginine N-methyltransferase 3 isoform 1, HRMT1L3, ATGP2958-10 µg, ATGP2958-20 µg, ATGP2958-50 µg, ATGP2958-100 µg, ATGP2958-250 µg, ATGP2958-500 µg, ATGP2958-1 mg, ATGP2958-10, ATGP2958-20, ATGP2958-50, ATGP2958-100, ATGP2958-250, ATGP2958-500, ATGP2958-1

Tang J, Gary JD, et al. (1998) . J Biol Chem. 273 (27) :16935-45.; ; Choi S, Jung CR, et al. (2008) . Biochim Biophys Acta. 1780 (9) :1062-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Signal Transduction

NP_005779.1

O60678

Human

MCSLASG ATGGRGAVEN EEDLPELSDS GDEAAWEDED DADLPHGKQQ TPCLFCNRLF TSAEETFSHC KSEHQFNIDS MVHKHGLEFY GYIKLINFIR LKNPTVEYMN SIYNPVPWEK EEYLKPVLED DLLLQFDVED LYEPVSVPFS YPNGLSENTS VVEKLKHMEA RALSAEAALA RAREDLQKMK QFAQDFVMHT DVRTCSSSTS VIADLQEDED GVYFSSYGHY GIHEEMLKDK IRTESYRDFI YQNPHIFKDK VVLDVGCGTG ILSMFAAKAG AKKVLGVDQS EILYQAMDII RLNKLEDTIT LIKGKIEEVH LPVEKVDVII SEWMGYFLLF ESMLDSVLYA KNKYLAKGGS VYPDICTISL VAVSDVNKHA DRIAFWDDVY GFKMSCMKKA VIPEAVVEVL DPKTLISEPC GIKHIDCHTT SISDLEFSSD FTLKITRTSM CTAIAGYFDI YFEKNCHNRV VFSTGPQSTK THWKQTVFLL EKPFSVKAGE ALKGKVTVHK NKKDPRSLTV TLTLNNSTQT YGLQ