Recombinant humaan PRND-eiwit

PRND is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. This protein is expressed during embryogenesis, but is expressed minimally in the central nervous system. Its function is still unknown. Mutations in PRND may lead to neurological disorders. Recombinant human PRND protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prion-like protein doppel, Prion protein 2, DOPPEL, DPL, PrPLP

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

16.9 kDa (149aa) confirmed by MALDI-TOF

PRND, Prion-like protein doppel, Prion protein 2, DOPPEL, DPL, PrPLP, ATGP1924-10 µg, ATGP1924-20 µg, ATGP1924-50 µg, ATGP1924-100 µg, ATGP1924-250 µg, ATGP1924-500 µg, ATGP1924-1 mg, ATGP1924-10, ATGP1924-20, ATGP1924-50, ATGP1924-100, ATGP1924-250, ATGP1924-500, ATGP1924-1

Peoc'h K, Guerin C, et al. (2000) . Neurosci Lett. 286 (2) :144-8.; ; Serres C, Peoc'h K, et al. (2006) . Biol Reprod. 74 (5) :816-23.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.2M NaCl, 30% glycerol, 1mM DTT

Neuroscience

NP_036541

Q9UKY0

Human

RGIKHRI KWNRKALPST AQITEAQVAE NRPGAFIKQG RKLDIDFGAE GNRYYEANYW QFPDGIHYNG CSEANVTKEA FVTGCINATQ AANQGEFQKP DNKLHQQVLW RLVQELCSLK HCEFWLERG