Recombinant humaan Pro-insuline-eiwit

INS, also known as insulin preproprotein, is a biologically inactive precursor to the biologically active endocrine hormone insulin. Insulin is an essential hormone for maintaining metabolic homeostasis in the body. To make fully bioactive insulin, pancreatic beta cells initiate synthesis of INS. It is converted into proinsulin by signal peptidases, which remove its signal peptide from its N-terminus. Finally, proinsulin is converted into the bioactive hormone insulin by removal of the C-peptide. Recombinant human INS, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography techniques.

Human Proinsulin in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4 µg/mL.

Insulin preproprotein, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured in a cell proliferation assay using MCF7 human breast cancer cell. The ED50 range ≤ 4 µg/mL.

11.8 kDa (109aa) confirmed by MALDI-TOF

Proinsulin, Insulin preproprotein, IDDM, IDDM1, IDDM2, ILPR, IRDN, MODY10, ATGP3817-10 µg, ATGP3817-20 µg, ATGP3817-50 µg, ATGP3817-100 µg, ATGP3817-250 µg, ATGP3817-500 µg, ATGP3817-1 mg, ATGP3817-10, ATGP3817-20, ATGP3817-50, ATGP3817-100, ATGP3817-250, ATGP3817-500, ATGP3817-1

Sun J., et al. (2015) Mol Aspects Med. 42:105-118.; ; Liu M., et al. (2014) Vitam Horm. 95:35-62.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_000198

P01308

Human

FVNQHLC GSHLVEALYL VCGERGFFYT PKTRREAEDL QVGQVELGGG PGAGSLQPLA LEGSLQKRGI VEQCCTSICS LYQLENYCN