Recombinant humaan Profiline 2/PFN2-eiwit

PFN2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. This protein is ubiquitously expressed with highest expression in kidney, brain and skeletal muscle. Like other members of the profilin family, PFN2 functions as an actin monomer-binding protein that influences the structure of the cytoskeleton by regulating actin polymerization in response to extracellular signals. At high concentrations, it prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Recombinant PFN2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Profilin 2, PFL, Profilin 2 D3S1319E, PFN 2, PFN2, Profilin II, ProfilinII, Profilin2

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.2 kDa (160aa) confirmed by MALDI-TOF

PFN2, Profilin 2, PFL, Profilin 2 D3S1319E, PFN 2, PFN2, Profilin II, ProfilinII, Profilin2, ATGP0535-10 µg, ATGP0535-20 µg, ATGP0535-50 µg, ATGP0535-100 µg, ATGP0535-250 µg, ATGP0535-500 µg, ATGP0535-1 mg, ATGP0535-10, ATGP0535-20, ATGP0535-50, ATGP0535-100, ATGP0535-250, ATGP0535-500, ATGP0535-1

Gieslmann R., et al. (1995) Eur J Bilchem. 229 (3) :621-8. ; ; Reinhard M., et al. (1995) EMBO J. 14 (8) :1583-9. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT

Cytoskeleton

AAH18049

P35080

Human

MAGWQSYVDN LMCDGCCQEA AIVGYCDAKY VWAATAGGVF QSITPIEIDM IVGKDREGFF TNGLALGAKK CSVIRDSLYV DGDCTMDIRT KSQGGEPTYN VAVGRAGRVL VFVMGKEGVH GGGLNKKAYS MAKYLRDSGF