Recombinant humaan prohibitine-eiwit

PHB is an evolutionarily conserved gene that is ubiquitously expressed. It is thought to be a negative regulator of cell proliferation and may be a tumor suppressor. Mutations in PHB have been linked to sporadic breast cancer. Prohibitin is expressed as two transcripts with varying lengths of 3' untranslated region. The longer transcript is present at higher levels in proliferating tissues and cells, suggesting that this longer 3' untranslated region may function as a trans-acting regulatory RNA. Recombinant human PHB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prohibitin, PHB1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

31.9 kDa (292aa) confirmed by MALDI-TOF

Prohibitin, Prohibitin, PHB1, ATGP1987-10 µg, ATGP1987-20 µg, ATGP1987-50 µg, ATGP1987-100 µg, ATGP1987-250 µg, ATGP1987-500 µg, ATGP1987-1 mg, ATGP1987-10, ATGP1987-20, ATGP1987-50, ATGP1987-100, ATGP1987-250, ATGP1987-500, ATGP1987-1

Van Aken O. et al. (2007) Plant J. 52:850-864.; ; Mishra S. et al. (2006) J Cell Mol Med. 10:353-363.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Metabolism

NP_002625.1

P35232

Human

MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA SQLPRIFTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ LSRSRNITYL PAGQSVLLQL PQ