Recombinant humaan Properdin-eiwit

CFP is a plasma glycoprotein that positively regulates the alternative complement pathway of the innate immune system. This protein binds to many microbial surfaces and apoptotic cells and stabilizes the C3- and C5-convertase enzyme complexes in a feedback loop that ultimately leads to formation of the membrane attack complex and lysis of the target cell. Mutations in this gene result in two forms of properdin deficiency, which results in high susceptibility to meningococcal infections. Multiple alternatively spliced variants, encoding the same protein, have been identified. Recombinant human CFP protein, fused to His-tag at N-terminus, was expressed in E. coli.

Complement factor properdin, BFD, PFC, PFD, PROPERDIN

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

50.9 kDa (465aa)

Properdin, Complement factor properdin, BFD, PFC, PFD, PROPERDIN, ATGP2745-10 µg, ATGP2745-20 µg, ATGP2745-50 µg, ATGP2745-100 µg, ATGP2745-250 µg, ATGP2745-500 µg, ATGP2745-1 mg, ATGP2745-10, ATGP2745-20, ATGP2745-50, ATGP2745-100, ATGP2745-250, ATGP2745-500, ATGP2745-1

Hartmann S., et al. (2000) J. Biol. Chem. 275:28569-28574; ; Liu T., et al. (2005) J. Proteome Res. 4:2070-2080;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Immunology

NP_002612

P27918

Human

MGSSHHHHHH SSGLVPRGSH MGSDPVLCFT QYEESSGKCK GLLGGGVSVE DCCLNTAFAY QKRSGGLCQP CRSPRWSLWS TWAPCSVTCS EGSQLRYRRC VGWNGQCSGK VAPGTLEWQL QACEDQQCCP EMGGWSGWGP WEPCSVTCSK GTRTRRRACN HPAPKCGGHC PGQAQESEAC DTQQVCPTHG AWATWGPWTP CSASCHGGPH EPKETRSRKC SAPEPSQKPP GKPCPGLAYE QRRCTGLPPC PVAGGWGPWG PVSPCPVTCG LGQTMEQRTC NHPVPQHGGP FCAGDATRTH ICNTAVPCPV DGEWDSWGEW SPCIRRNMKS ISCQEIPGQQ SRGRTCRGRK FDGHRCAGQQ QDIRHCYSIQ HCPLKGSWSE WSTWGLCMPP CGPNPTRARQ RLCTPLLPKY PPTVSMVEGQ GEKNVTFWGR PLPRCEELQG QKLVVEEKRP CLHVPACKDP EEEEL