Recombinant humaan Prostaatzuur Fosfatase/ACS-eiwit

ACPP, also known as prostatic acid phosphatase isoform PAP, is a type I integral membrane protein of the plasma membrane and lysosomes, and a secreted form also exists. The concentration of ACPP is elevated in the circulation of prostate cancer patients, making the enzyme a marker for the progression of prostate cancer. Recombinant human ACPP, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 100,000 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Prostatic acid phosphatase isoform PAP, ACPP, 5-NT, ACP-3, ACP3, 5-NT, 5-nucleotidase, Acid phosphatase prostate, ACP 3, ACP3, acpP Ecto-5-nucleotidase, PAP, PPAP_HUMAN, Prostatic acid phosphatase, Thiamine monophosphatase, TMPase

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 100,000unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

41.8 kDa (360aa)

Prostatic Acid Phosphatase, ACPP, Prostatic acid phosphatase isoform PAP , ACPP, 5-NT, ACP-3, ACP3, 5-NT, 5-nucleotidaseAcid phosphatase prostate, ACP 3, ACP3, acpPEcto-5-nucleotidase, PAP, PPAP_HUMANProstatic acid phosphatase, Thiamine monophosphatase, TMPase, ATGP3390-10 µg, ATGP3390-20 µg, ATGP3390-50 µg, ATGP3390-100 µg, ATGP3390-250 µg, ATGP3390-500 µg, ATGP3390-1 mg, ATGP3390-10, ATGP3390-20, ATGP3390-50, ATGP3390-100, ATGP3390-250, ATGP3390-500, ATGP3390-1

Chuang TD., et al. (2010) J Biol Chem. 285:23598-23606.; ; Taira A., et al. (2007) Oncology. 21:1003-1010.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cancer

NP_001090.2

P15309

Human

Lys33