Recombinant humaan proteïneremmer/SERPINA5 eiwit

Serpin A5, also known as PCI (Protein C Inhibitor) or PAI3 (plasminogen activator inhibitor 3), is a member of the serpin serine proteinase inhibitor family. This protein inhibits plasminogen activators as well as activated protein C. Serpin A5 is secreted in plasma, but is also expressed in liver. Serpin A5 is involved in cell inflammation, proliferation, apoptosis, tumor cell migration, invasion, and metastasis. Recombinant Serpin A5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 2 nM

Serpin peptidase inhibitor, clade A, member 5, PAI3, PCI, PROCI, Plasma serine protease inhibitor, Acrosomal serine protease inhibitor, Plasminogen activator inhibitor 3, Protein C inhibitor, SERPINA5, PLANH3, Serine proteinase inhibitor clade A (alpha-1 antiproteinase, antitrypsin) member 5, Cysteine proteinase inhibitor clade A (alpha-1 antiproteinase, antitrypsin) member 5

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Measured by its ability to inhibit Thrombin cleavage of substrate Boc-VPR-AMC. The IC50 for this effect is less or equal to 2 nM

45.9 kDa (408aa) confirmed by MALDI-TOF

Serpin A5, Serpin peptidase inhibitor, clade A, member 5, PAI3, PCI, PROCI, ATGP3746-10 µg, ATGP3746-20 µg, ATGP3746-50 µg, ATGP3746-100 µg, ATGP3746-250 µg, ATGP3746-500 µg, ATGP3746-1 mg, ATGP3746-10, ATGP3746-20, ATGP3746-50, ATGP3746-100, ATGP3746-250, ATGP3746-500, ATGP3746-1

Han MH., et al. (2008) Nature. 451 (7182) :1076-81. ; ; Li W., et al. (2008) J Biol Chem. 283 (51) :36039-45.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM MES buffer (pH 6.0) containing 150 mM NaCl, 10% glycerol

Cardiovascular

AAH08915

P05154

Human

MGSSHHHHHH SSGLVPRGSH MHRHHPREMK KRVEDLHVGA TVAPSSRRDF TFDLYRALAS AAPSQNIFFS PVSISMSLAM LSLGAGSSTK MQILEGLGLN LQKSSEKELH RGFQQLLQEL NQPRDGFQLS LGNALFTDLV VDLQDTFVSA MKTLYLADTF PTNFRDSAGA MKQINDYVAK QTKGKIVDLL KNLDSNAVVI MVNYIFFKAK WETSFNHKGT QEQDFYVTSE TVVRVPMMSR EDQYHYLLDR NLSCRVVGVP YQGNATALFI LPSEGKMQQV ENGLSEKTLR KWLKMFKKRQ LELYLPKFSI EGSYQLEKVL PSLGISNVFT SHADLSGISN HSNIQVSEMV HKAVVEVDES GTRAAAATGT IFTFRSARLN SQRLVFNRPF LMFIVDNNIL FLGKVNRP