Recombinant humaan PRPS1-eiwit

PRPS1, also known as ribose-phosphate pyrophosphokinase 1, is ubiquitously expressed in human tissues. This enzyme catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for purine metabolism and nucleotide biosynthesis. Recombinant human PRPS1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1, Ribose-phosphate pyrophosphokinase 1 EC 2.7.6.1, KIAA0967, Phosphoribosyl pyrophosphate synthetase I, PRS I, PRS-I, Ribose phosphate pyrophosphokinase I

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

36.9 kDa (338aa) confirmed by MALDI-TOF

PRPS1, Ribose-phosphate pyrophosphokinase 1, ARTS, CMTX5, PPRibP, PRSI, DFN2, DFNX1, Ribose-phosphate pyrophosphokinase 1 EC 2.7.6.1, KIAA0967, Phosphoribosyl pyrophosphate synthetase I, PRS I, PRS-I, Ribose phosphate pyrophosphokinase I, ATGP0460-10 µg, ATGP0460-20 µg, ATGP0460-50 µg, ATGP0460-100 µg, ATGP0460-250 µg, ATGP0460-500 µg, ATGP0460-1 mg, ATGP0460-10, ATGP0460-20, ATGP0460-50, ATGP0460-100, ATGP0460-250, ATGP0460-500, ATGP0460-1

Liu H., et al. (2009) Biochem Biophys Res Commun. 379 (4) :1120-5. ; ; Li S., et al. (2007) Biochem J. 401 (1) :39-47.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1 M NaCl, and 20% glycerol

Enzymes & Proteases

NP_002755

P60891

Human

MPNIKIFSGS SHQDLSQKIA DRLGLELGKV VTKKFSNQET CVEIGESVRG EDVYIVQSGC GEINDNLMEL LIMINACKIA SASRVTAVIP CFPYARQDKK DKSRAPISAK LVANMLSVAG ADHIITMDLH ASQIQGFFDI PVDNLYAEPA VLKWIRENIS EWRNCTIVSP DAGGAKRVTS IADRLNVDFA LIHKERKKAN EVDRMVLVGD VKDRVAILVD DMADTCGTIC HAADKLLSAG ATRVYAILTH GIFSGPAISR INNACFEAVV VTNTIPQEDK MKHCSKIQVI DISMILAEAI RRTHNGESVS YLFSHVPL