Recombinant humaan PRRT2-eiwit

PRRT2 is a transmembrane protein containing a proline-rich domain in its N-terminal half. Studies in mice suggest that it is predominantly expressed in brain and spinal cord in embryonic and postnatal stages. Mutations in this gene are associated with paroxysmal kinesigenic dyskinesia. Almost one third of sporadic PKC patients also carry PRRT2 mutations. Recombinant human PRRT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proline-rich transmembrane protein 2 isoform 3, BFIC2, BFIS2, DSPB3, DYT10, EKD1, ICCA, IFITMD1, PKC

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

29.7 kDa (291aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PRRT2, Proline-rich transmembrane protein 2 isoform 3, BFIC2, BFIS2, DSPB3, DYT10, EKD1, ICCA, IFITMD1, PKC, ATGP2531-10 µg, ATGP2531-20 µg, ATGP2531-50 µg, ATGP2531-100 µg, ATGP2531-250 µg, ATGP2531-500 µg, ATGP2531-1 mg, ATGP2531-10, ATGP2531-20, ATGP2531-50, ATGP2531-100, ATGP2531-250, ATGP2531-500, ATGP2531-1

Li J, Zhu X, Wang X, et al. (2012) . J Med Genet. 49 (2) :76-8. ; ; Chen WJ, Lin Y, et al. (2011) . Nat Genet. 43 (12) :1252-5.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Other

NP_001243372

Q7Z6L0

Human

MAASSSE ISEMKGVEES PKVPGEGPGH SEAETGPPQV LAGVPDQPEA PQPGPNTTAA PVDSGPKAGL APETTETPAG ASETAQATDL SLSPGGESKA NCSPEDPCQE TVSKPEVSKE ATADQGSRLE SAAPPEPAPE PAPQPDPRPD SQPTPKPALQ PELPTQEDPT PEILSESVGE KQENGAVVPL QAGDGEEGPA PEPHSPPSKK SPPANGAPPR VLQQLVEEDR MRRAHSGHPG SPRGSLSRHP SSQLAGPGVE GGEGTQKPRD Y