Recombinant humaan PSAT1-eiwit

PSAT1, also known as phosphoserine aminotransferase, catalyses the conversion of 3-phosphohydroxypyruvate into 3-phosphoserine which is dephosphorylated subsequently by phosphoserine phosphatase to form L-serine. Recombinant human PSAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Phosphoserine aminotransferase, EPIP, PSA, PSAT

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.9 kDa (394aa) confirmed by MALDI-TOF

PSAT1, Phosphoserine aminotransferase, EPIP, PSA, PSAT, ATGP1328-10 µg, ATGP1328-20 µg, ATGP1328-50 µg, ATGP1328-100 µg, ATGP1328-250 µg, ATGP1328-500 µg, ATGP1328-1 mg, ATGP1328-10, ATGP1328-20, ATGP1328-50, ATGP1328-100, ATGP1328-250, ATGP1328-500, ATGP1328-1

Choudhary C., et al. (2009) Science. 325:834-840; ; Hart C.E., et al. (2007) Am. J. Hum. Genet. 80:931-937

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Metabolism

NP_478059

Q9Y617

Human

MDAPRQ VVNFGPGPAK LPHSVLLEIQ KELLDYKGVG ISVLEMSHRS SDFAKIINNT ENLVRELLAV PDNYKVIFLQ GGGCGQFSAV PLNLIGLKAG RCADYVVTGA WSAKAAEEAK KFGTINIVHP KLGSYTKIPD PSTWNLNPDA SYVYYCANET VHGVEFDFIP DVKGAVLVCD MSSNFLSKPV DVSKFGVIFA GAQKNVGSAG VTVVIVRDDL LGFALRECPS VLEYKVQAGN SSLYNTPPCF SIYVMGLVLE WIKNNGGAAA MEKLSSIKSQ TIYEIIDNSQ GFYVCPVEPQ NRSKMNIPFR IGNAKGDDAL EKRFLDKALE LNMLSLKGHR SVGGIRASLY NAVTIEDVQK LAAFMKKFLE MHQL