Recombinant humaan PSGL-1/CD162-eiwit

PSGL-1, also known CD162 or SELPLG, is a mucin-type glycoprotein that functions as a high affinity counter-receptor for the cell adhesion molecules P-, Eand L- selectin expressed on myeloid cells and stimulated T lymphocytes. This protein plays a key role in leukocyte adhesion. As such, it plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. PSGL-1 binds chmokines such as CCL19, CCL21, and CCL27, promoting chemotaxis of hematopoietic stem cells and plasma cells to the bone marrow and T cell homing to lymphoid organs. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. Recombinant human PSGL-1, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

SELPLG, CLA, CD162, P-selectin glycoprotein ligand 1, PSGL1, Selectin P ligand, PSGL-1, ligand for P-slectin

HEK293

Human

HIgG-His-Tag

SDS-PAGE

1 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

53.4kDa (496aa)

SELPLG, ATGP3999-10 µg, ATGP3999-20 µg, ATGP3999-50 µg, ATGP3999-100 µg, ATGP3999-250 µg, ATGP3999-500 µg, ATGP3999-1 mg, ATGP3999-10, ATGP3999-20, ATGP3999-50, ATGP3999-100, ATGP3999-250, ATGP3999-500, ATGP3999-1

Carlisle R., et al, (2013) Pharm Res. 30:352-361.; ; Tinoco R., et al, (2016) Immunity. 44:1190-1203.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology, Adhesion & Movement

AAC50061.1

Q14242

Human

Asp

QATEYEY LDYDFLPETE PPEMLRNSTD TTPLTGPGTP ESTTVEPAAR RSTGLDAGGA VTELTTELAN MGNLSTDSAA MEIQTTQPAA TEAQTTPLAA TEAQTTRLTA TEAQTTPLAA TEAQTTPPAA TEAQTTQPTG LEAQTTAPAA MEAQTTAPAA MEAQTTPPAA MEAQTTQTTA MEAQTTAPEA TEAQTTQPTA TEAQTTPLAA MEALSTEPSA TEALSMEPTT KRGLFIPFSV SSVTHKGIPM AASNLSV