Recombinant humaan PSMA1-eiwit

PSMA1 is a prosomal protein involved in a nonlysosomal ATP/ubiquitin-dependent proteolytic pathway. The protein is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. This protein highly expressed in prostate epithelium. Recombinant human PSMA1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit alpha type-1 isoform 2, HC2, Nu, PROS30

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32 kDa (286aa) confirmed by MALDI-TOF

PSMA1, Proteasome subunit alpha type-1 isoform 2, HC2, Nu, PROS30, ATGP1241-10 µg, ATGP1241-20 µg, ATGP1241-50 µg, ATGP1241-100 µg, ATGP1241-250 µg, ATGP1241-500 µg, ATGP1241-1 mg, ATGP1241-10, ATGP1241-20, ATGP1241-50, ATGP1241-100, ATGP1241-250, ATGP1241-500, ATGP1241-1

DeMartino GN., et al. (1991) Biochim Biophys Acta. 1079 (1) :29-38.; ; Silva Pereira I., et al. (1992) Gene. 120 (2) :235-42.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.15M NaCl.

Cell Cycle

NP_002777

P25786

Human

MFRNQYD NDVTVWSPQG RIHQIEYAME AVKQGSATVG LKSKTHAVLV ALKRAQSELA AHQKKILHVD NHIGISIAGL TADARLLCNF MRQECLDSRF VFDRPLPVSR LVSLIGSKTQ IPTQRYGRRP YGVGLLIAGY DDMGPHIFQT CPSANYFDCR AMSIGARSQS ARTYLERHMS EFMECNLNEL VKHGLRALRE TLPAEQDLTT KNVSIGIVGK DLEFTIYDDD DVSPFLEGLE ERPQRKAQPA QPADEPAEKA DEPMEH