Recombinant humaan PSMA3-eiwit

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that is a 20S core alpha subunit. Two alternative transcripts encoding different isoforms have been identified. Recombinant human PSMA3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome (prosome macropain) subunit alpha type 3, Proteasome (prosome, macropain) subunit, alpha type 3, HC8, MGC12306, MGC32631, PSC3

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

30.5 kDa (275aa) confirmed by MALDI-TOF

PSMA3, Proteasome (prosome macropain) subunit alpha type 3, Proteasome (prosome, macropain) subunit, alpha type 3, HC8, MGC12306, MGC32631, PSC3, ATGP1287-10 µg, ATGP1287-20 µg, ATGP1287-50 µg, ATGP1287-100 µg, ATGP1287-250 µg, ATGP1287-500 µg, ATGP1287-1 mg, ATGP1287-10, ATGP1287-20, ATGP1287-50, ATGP1287-100, ATGP1287-250, ATGP1287-500, ATGP1287-1

Kristensen P., et al. (1995) Biochem Biophys Res Commun. 207 (3) :1059.; ; Wenzel T., et al. (1995) Nat Struct Biol. 2 (3) :199-204.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Ubiquitination

NP_002779

P25788

Human

MSSIGTGYDL SASTFSPDGR VFQVEYAMKA VENSSTAIGI RCKDGVVFGV EKLVLSKLYE EGSNKRLFNV DRHVGMAVAG LLADARSLAD IAREEASNFR SNFGYNIPLK HLADRVAMYV HAYTLYSAVR PFGCSFMLGS YSVNDGAQLY MIDPSGVSYG YWGCAIGKAR QAAKTEIEKL QMKEMTCRDI VKEVAKIIYI VHDEVKDKAF ELELSWVGEL TNGRHEIVPK DIREEAEKYA KESLKEEDES DDDNM