Recombinant humaan PSMA4-eiwit

PSMA4, also known as proteasome subunit alpha type 4, is a member of the peptidase T1A family, that is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Proteasome subunit alpha type-4 isoform 1, HC9, HsT17706, MGC111191, MGC12467, MGC24813, PSC9

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.0 kDa (285aa) confirmed by MALDI-TOF

PSMA4, Proteasome subunit alpha type-4 isoform 1, HC9, HsT17706, MGC111191, MGC12467, MGC24813, PSC9, ATGP1350-10 µg, ATGP1350-20 µg, ATGP1350-50 µg, ATGP1350-100 µg, ATGP1350-250 µg, ATGP1350-500 µg, ATGP1350-1 mg, ATGP1350-10, ATGP1350-20, ATGP1350-50, ATGP1350-100, ATGP1350-250, ATGP1350-500, ATGP1350-1

Kristensen P. et al. (1995) Biochem. Biophys. Res.Commun. 205: 1785-1789.; ; Morimoto Y. et al. (1995) J. Biochem. 117: 471-474

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT, 0.1mM PMSF

Enzymes & Proteases

NP_001096137

P25789

Human

MGSSHHHHHH SSGLVPRGSH MGSHMSRRYD SRTTIFSPEG RLYQVEYAME AIGHAGTCLG ILANDGVLLA AERRNIHKLL DEVFFSEKIY KLNEDMACSV AGITSDANVL TNELRLIAQR YLLQYQEPIP CEQLVTALCD IKQAYTQFGG KRPFGVSLLY IGWDKHYGFQ LYQSDPSGNY GGWKATCIGN NSAAAVSMLK QDYKEGEMTL KSALALAIKV LNKTMDVSKL SAEKVEIATL TRENGKTVIR VLKQKEVEQL IKKHEEEEAK AEREKKEKEQ KEKDK