Recombinant humaan PSMA5 eiwit

PSMA5 (Proteasome subunit alpha type-5) belongs to the peptidase T1A family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA5 has been shown to interact with PLK1. This peptidase has a very broad specificity for cleavage of peptide bonds. Recombinant human PSMA5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit alpha type-5, PSC5, ZETA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30.5 kDa (277aa) confirmed by MALDI-TOF

PSMA5, Proteasome subunit alpha type-5, PSC5, ZETA, ATGP1336-10 µg, ATGP1336-20 µg, ATGP1336-50 µg, ATGP1336-100 µg, ATGP1336-250 µg, ATGP1336-500 µg, ATGP1336-1 mg, ATGP1336-10, ATGP1336-20, ATGP1336-50, ATGP1336-100, ATGP1336-250, ATGP1336-500, ATGP1336-1

Feng, Y., et al. (2001) Cell Growth Differ. (united States) 12 (1) : 29-37.; ; Hirano Y., et al. (2005) Nature 437:1381-1385

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 10% glycerol, 100 mM NaCl

Enzymes & Proteases

NP_002781

P28066

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMFLT RSEYDRGVNT FSPEGRLFQV EYAIEAIKLG STAIGIQTSE GVCLAVEKRI TSPLMEPSSI EKIVEIDAHI GCAMSGLIAD AKTLIDKARV ETQNHWFTYN ETMTVESVTQ AVSNLALQFG EEDADPGAMS RPFGVALLFG GVDEKGPQLF HMDPSGTFVQ CDARAIGSAS EGAQSSLQEV YHKSMTLKEA IKSSLIILKQ VMEEKLNATN IELATVQPGQ NFHMFTKEEL EEVIKDI