Recombinant humaan PSMA6-eiwit

Proteasome subunit alpha type 6, also known as PSMA6, is a member of the peptidase T1A family, which is a 20S core alpha subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. PSMA6 is distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMA6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit alpha type 6, IOTA, p27K, PROS27

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.9 kDa (270aa) confirmed by MALDI-TOF

PSMA6, Proteasome subunit alpha type 6, IOTA, p27K, PROS27, ATGP1308-10 µg, ATGP1308-20 µg, ATGP1308-50 µg, ATGP1308-100 µg, ATGP1308-250 µg, ATGP1308-500 µg, ATGP1308-1 mg, ATGP1308-10, ATGP1308-20, ATGP1308-50, ATGP1308-100, ATGP1308-250, ATGP1308-500, ATGP1308-1

Nandi D., et al. (2006) J Biosci. 31:137-155.; ; Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl

Ubiquitination

NP_002782

P60900

Human

MSRGSS AGFDRHITIF SPEGRLYQVE YAFKAINQGG LTSVAVRGKD CAVIVTQKKV PDKLLDSSTV THLFKITENI GCVMTGMTAD SRSQVQRARY EAANWKYKYG YEIPVDMLCK RIADISQVYT QNAEMRPLGC CMILIGIDEE QGPQVYKCDP AGYYCGFKAT AAGVKQTEST SFLEKKVKKK FDWTFEQTVE TAITCLSTVL SIDFKPSEIE VGVVTVENPK FRILTEAEID AHLVALAERD