Recombinant humaan PSMA8-eiwit

Proteasome subunit alpha type-8, also known as PSMA8, belongs to the peptidase T1A family. PSMA8 is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This subunit may be a testis-specific. Recombinant human PSMA8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit alpha type-8, PSMA7L

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

30 kDa (270aa) confirmed by MALDI-TOF

PSMA8, Proteasome subunit alpha type-8, PSMA7L, ATGP1320-10 µg, ATGP1320-20 µg, ATGP1320-50 µg, ATGP1320-100 µg, ATGP1320-250 µg, ATGP1320-500 µg, ATGP1320-1 mg, ATGP1320-10, ATGP1320-20, ATGP1320-50, ATGP1320-100, ATGP1320-250, ATGP1320-500, ATGP1320-1

Schmidt M., et al. (1997) Mal Biol Rep. 24:103-112.; ; Kristensen P., et al. (1995) Biochem Biophys Res Commun. 205:1785-1789.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_001020267

Q8TAA3

Human

MGSSHHHHHH SSGLVPRGSH MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK SVAKLQDERT VRKICALDDH VCMAFAGLTA DARVVINRAR VECQSHKLTV EDPVTVEYIT RFIATLKQKY TQSNGRRPFG ISALIVGFDD DGISRLYQTD PSGTYHAWKA NAIGRSAKTV REFLEKNYTE DAIASDSEAI KLAIKALLEV VQSGGKNIEL AIIRRNQPLK MFSAKEVELY VTEIEKEKEE AEKKKSKKSV