Recombinant humaan PSMB10/MECL1 eiwit

PSMB10 is a member of the proteasome B-type family, also known as the T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Expression of the PSMB10 gene is induced by gamma interferon, and PSMB10 replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. Recombinant human PSMB10 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Proteasome subunit beta type-10, LMP10, MECL1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

26.9 kDa (255aa) confirmed by MALDI-TOF

PSMB10, MECL1, Proteasome subunit beta type-10, LMP10, MECL1, ATGP1212-10 µg, ATGP1212-20 µg, ATGP1212-50 µg, ATGP1212-100 µg, ATGP1212-250 µg, ATGP1212-500 µg, ATGP1212-1 mg, ATGP1212-10, ATGP1212-20, ATGP1212-50, ATGP1212-100, ATGP1212-250, ATGP1212-500, ATGP1212-1

Foss G.S., et al. (1998) Biochim. Biophys. Acta. 1402:17-28; ; Hisamatsu H., et al. (1996) J. Exp. Med. 183:1807-1816

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl

Immunology

NP_002792.1

P40306

Human

TTIAGLVFQ DGVILGADTR ATNDSVVADK SCEKIHFIAP KIYCCGAGVA ADAEMTTRMV ASKMELHALS TGREPRVATV TRILRQTLFR YQGHVGASLI VGGVDLTGPQ LYGVHPHGSY SRLPFTALGS GQDAALAVLE DRFQPNMTLE AAQGLLVEAV TAGILGDLGS GGNVDACVIT KTGAKLLRTL SSPTEPVKRS GRYHFVPGTT AVLTQTVKPL TLELVEETVQ AMEVE