Recombinant humaan PSMB2-eiwit

Proteasome subunit beta type-2, also known as PSMB2, is a member of the proteasome T1B family, which is a 20S core beta subunit. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. Recombinant human PSMB2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit beta type-2, HC7-I

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.9 kDa (221aa) confirmed by MALDI-TOF

PSMB2, Proteasome subunit beta type-2, HC7-I, ATGP1321-10 µg, ATGP1321-20 µg, ATGP1321-50 µg, ATGP1321-100 µg, ATGP1321-250 µg, ATGP1321-500 µg, ATGP1321-1 mg, ATGP1321-10, ATGP1321-20, ATGP1321-50, ATGP1321-100, ATGP1321-250, ATGP1321-500, ATGP1321-1

Morimoto Y., et al. (1995) J Biochem. 117:471-474.; ; Wnzel T., et al. (1995) Nat Struct Biol. 2:199-204.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Enzymes & Proteases

NP_002785

P49721

Human

MGSSHHHHHH SSGLVPRGSH MEYLIGIQGP DYVLVASDRV AASNIVQMKD DHDKMFKMSE KILLLCVGEA GDTVQFAEYI QKNVQLYKMR NGYELSPTAA ANFTRRNLAD CLRSRTPYHV NLLLAGYDEH EGPALYYMDY LAALAKAPFA AHGYGAFLTL SILDRYYTPT ISRERAVELL RKCLEELQKR FILNLPTFSV RIIDKNGIHD LDNISFPKQG S