Recombinant humaan PSMB3-eiwit

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides.. Recombinant human PSMB3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit beta type-3, HC10-II

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.1 kDa (225aa) confirmed by MALDI-TOF

PSMB3, Proteasome subunit beta type-3, HC10-II, ATGP1305-10 µg, ATGP1305-20 µg, ATGP1305-50 µg, ATGP1305-100 µg, ATGP1305-250 µg, ATGP1305-500 µg, ATGP1305-1 mg, ATGP1305-10, ATGP1305-20, ATGP1305-50, ATGP1305-100, ATGP1305-250, ATGP1305-500, ATGP1305-1

Huang L., et al. (2006) Meth Enzymil. 405:187-236.; ; Madding L S., et al. (2006) J Bacteriol. 189:583-590.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 50% glycerol, 0.2M NaCl

Ubiquitination

NP_002786.2

P49720

Human

MSIMSYNGGA VMAMKGKNCV AIAADRRFGI QAQMVTTDFQ KIFPMGDRLY IGLAGLATDV QTVAQRLKFR LNLYELKEGR QIKPYTLMSM VANLLYEKRF GPYYTEPVIA GLDPKTFKPF ICSLDLIGCP MVTDDFVVSG TCAEQMYGMC ESLWEPNMDP DHLFETISQA MLNAVDRDAV SGMGVIVHII EKDKITTRTL KARMD