Recombinant humaan PSMB4-eiwit

Proteasome subunit beta type-4, also known as PSMB4, belonged to the peptidase T1B family. The 20S Proteasome chamber contains alpha subunits (which are structural) and beta subunits (which are predominantly catalytic) . The outer two rings in the proteasome consist of seven alpha subunits each and the inner two rings each consist of seven beta subunits. PSMB4 is a beta subunit of the 20S Proteasome. It is distributed throughout eukaryotic cells at a high concentration and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. Recombinant human PSMB4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit beta type-4, HN3, HsN3, PROS-26, PROS26

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.6 kDa (240aa) confirmed by MALDI-TOF

PSMB4, Proteasome subunit beta type-4, HN3, HsN3, PROS-26, PROS26, ATGP1306-10 µg, ATGP1306-20 µg, ATGP1306-50 µg, ATGP1306-100 µg, ATGP1306-250 µg, ATGP1306-500 µg, ATGP1306-1 mg, ATGP1306-10, ATGP1306-20, ATGP1306-50, ATGP1306-100, ATGP1306-250, ATGP1306-500, ATGP1306-1

Orlowski M., et al. (1997) Biochemistry. 36:13946-13953.; ; McCusker D., et al. (1997) Genomics. 45:362-367.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Ubiquitination

NP_002787

P28070

Human

MGSSHHHHHH SSGLVPRGSH MTQNPMVTGT SVLGVKFEGG VVIAADMLGS YGSLARFRNI SRIMRVNNST MLGASGDYAD FQYLKQVLGQ MVIDEELLGD GHSYSPRAIH SWLTRAMYSR RSKMNPLWNT MVIGGYADGE SFLGYVDMLG VAYEAPSLAT GYGAYLAQPL LREVLEKQPV LSQTEARDLV ERCMRVLYYR DARSYNRFQI ATVTEKGVEI EGPLSTETNW DIAHMISGFE