Recombinant humaan PSMB5 eiwit

PSMB5 is a member of the proteasome B-type family that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit) . PSMB5 has an ATP dependent proteolytic activity and involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway. Recombinant human PSMB5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome (prosome macropain) subunit beta type 5., LMPX, MB1, MGC104214, X.

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

26.7 kDa (241aa) confirmed by MALDI-TOF

PSMB5, Proteasome (prosome macropain) subunit beta type 5., LMPX, MB1, MGC104214, X., ATGP0850-10 µg, ATGP0850-20 µg, ATGP0850-50 µg, ATGP0850-100 µg, ATGP0850-250 µg, ATGP0850-500 µg, ATGP0850-1 mg, ATGP0850-10, ATGP0850-20, ATGP0850-50, ATGP0850-100, ATGP0850-250, ATGP0850-500, ATGP0850-1

Coux O., et al. (1996) Annu Rev Biochem. 65:801-47. ; ; Wenzel T., et al. (1995) Nat Struct Biol. 2 (3) :199-204.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 50% glycerol, 1mM DTT, 0.15M NaCl

Cell Cycle

NP_002788

P28074

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P