Recombinant humaan PSMB6-eiwit

PSMB6 is a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by PSMB9. The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMB6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proteasome subunit beta type-6 precursor, DELTA, LMPY, MGC5169

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

24.2 kDa (226aa) confirmed by MALDI-TOF

PSMB6, Proteasome subunit beta type-6 precursor, DELTA, LMPY, MGC5169, ATGP1351-10 µg, ATGP1351-20 µg, ATGP1351-50 µg, ATGP1351-100 µg, ATGP1351-250 µg, ATGP1351-500 µg, ATGP1351-1 mg, ATGP1351-10, ATGP1351-20, ATGP1351-50, ATGP1351-100, ATGP1351-250, ATGP1351-500, ATGP1351-1

Apcher GS., et al. (2003) FEBS Lett. 553 (1-2) :200-4.; ; Stelzl u., et al. (2002) Cell. 122 (6) :957-68.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Enzymes & Proteases

NP_002789

P28072

Human

MGSSHHHHHH SSGLVPRGSH MTTIMAVQFD GGVVLGADSR TTTGSYIANR VTDKLTPIHD RIFCCRSGSA ADTQAVADAV TYQLGFHSIE LNEPPLVHTA ASLFKEMCYR YREDLMAGII IAGWDPQEGG QVYSVPMGGM MVRQSFAIGG SGSSYIYGYV DATYREGMTK EECLQFTANA LALAMERDGS SGGVIRLAAI AESGVERQVL LGDQIPKFAV ATLPPA