Recombinant humaan PSMB7 eiwit

PSMB7, also known as proteasome subunit beta type-7, is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family that is a 20S core beta subunit in the proteasome. Expression of this catalytic subunit is downregulated by gamma interferon and proteolytic processing is required to generate a mature subunit. This subunit is not present in the immunoproteasome and is replaced by catalytic subunit 2i. Recombinant human PSMB7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, Proteasome subunit beta type 7

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

27.6 kDa (255aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

PSMB7, Proteasome subunit beta type-7, Macropain chain Z, Multicatalytic endopeptidase complex chain Z, Proteasome subunit Z, Proteasome subunit beta type 7, ATGP1426-10 µg, ATGP1426-20 µg, ATGP1426-50 µg, ATGP1426-100 µg, ATGP1426-250 µg, ATGP1426-500 µg, ATGP1426-1 mg, ATGP1426-10, ATGP1426-20, ATGP1426-50, ATGP1426-100, ATGP1426-250, ATGP1426-500, ATGP1426-1

Wang X., et al. (2007) Biochemistry. 46:3553-3565; ; Rho J.H., et al. (2008) J. Proteome Res. 7:2959-2972

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_002790

Q99436

Human

TTIAGVVYK DGIVLGADTR ATEGMVVADK NCSKIHFISP NIYCCGAGTA ADTDMTTQLI SSNLELHSLS TGRLPRVVTA NRMLKQMLFR YQGYIGAALV LGGVDVTGPH LYSIYPHGST DKLPYVTMGS GSLAAMAVFE DKFRPDMEEE EAKNLVSEAI AAGIFNDLGS GSNIDLCVIS KNKLDFLRPY TVPNKKGTRL GRYRCEKGTT AVLTEKITPL EIEVLEETVQ TMDTS