Recombinant humaan PSMD11-eiwit

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. PSMD11 is a member of the proteasome subunit S9 family that functions as a non-ATPase subunit of the 19S regulator and is phosphorylated by AMP-activated protein kinase. Recombinant human PSMD11 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Proteasome 26S non-ATPase subunit 11, S9, Rpn6, p44.5, MGC3844

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

49.6 kDa (442aa)

PSMD11, proteasome 26S non-ATPase subunit 11, S9, Rpn6, p44.5, MGC3844, ATGP2470-10 µg, ATGP2470-20 µg, ATGP2470-50 µg, ATGP2470-100 µg, ATGP2470-250 µg, ATGP2470-500 µg, ATGP2470-1 mg, ATGP2470-10, ATGP2470-20, ATGP2470-50, ATGP2470-100, ATGP2470-250, ATGP2470-500, ATGP2470-1

Vilchez D., et al (2012) . Nature 489:304-308; ; Moreno D., et al (2009) . Int. J. Biochem. Cell Biol. 41:2431-2439;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Ubiquitination

NP_002806

O00231

Human

MGSSHHHHHH SSGLVPRGSH MAAAAVVEFQ RAQSLLSTDR EASIDILHSI VKRDIQENDE EAVQVKEQSI LELGSLLAKT GQAAELGGLL KYVRPFLNSI SKAKAARLVR SLLDLFLDME AATGQEVELC LECIEWAKSE KRTFLRQALE ARLVSLYFDT KRYQEALHLG SQLLRELKKM DDKALLVEVQ LLESKTYHAL SNLPKARAAL TSARTTANAI YCPPKLQATL DMQSGIIHAA EEKDWKTAYS YFYEAFEGYD SIDSPKAITS LKYMLLCKIM LNTPEDVQAL VSGKLALRYA GRQTEALKCV AQASKNRSLA DFEKALTDYR AELRDDPIIS THLAKLYDNL LEQNLIRVIE PFSRVQIEHI SSLIKLSKAD VERKLSQMIL DKKFHGILDQ GEGVLIIFDE PPVDKTYEAA LETIQNMSKV VDSLYNKAKK LT