Recombinant humaan PSMD9-eiwit

PSMD9 acts as a chaperone during the assembly of the 26S proteasome, specifically of the base subcomplex of the PA700/19S regulatory complex (RC) . During the base subcomplex assembly is part of an intermediate PSMD9:PSMC6:PSMC3 module, also known as modulator trimer complex; PSMD9 is released during the further base assembly process. Recombinant human PSMD9 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.1 kDa (246aa) confirmed by MALDI-TOF

PSMD9, 26S proteasome non-ATPase regulatory subunit 9, p27, Rpn4, ATGP1804-10 µg, ATGP1804-20 µg, ATGP1804-50 µg, ATGP1804-100 µg, ATGP1804-250 µg, ATGP1804-500 µg, ATGP1804-1 mg, ATGP1804-10, ATGP1804-20, ATGP1804-50, ATGP1804-100, ATGP1804-250, ATGP1804-500, ATGP1804-1

Kaneko T., et al. (2009) Cell. 137:914-925; ; Watanabe T.K., et al. (1998) Genomics. 50:241-250;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Ubiquitination

NP_002804

O00233

Human

MGSSHHHHHH SSGLVPRGSH MGSMSDEEAR QSGGSSQAGV VTVSDVQELM RRKEEIEAQI KANYDVLESQ KGIGMNEPLV DCEGYPRSDV DLYQVRTARH NIICLQNDHK AVMKQVEEAL HQLHARDKEK QARDMAEAHK EAMSRKLGQS ESQGPPRAFA KVNSISPGSP ASIAGLQVDD EIVEFGSVNT QNFQSLHNIG SVVQHSEGKP LNVTVIRRGE KHQLRLVPTR WAGKGLLGCN IIPLQR