Recombinant humaan PSMF1-eiwit

PSMF1 is a protein that inhibits the activation of the proteasome by the 11S and 19S regulators. The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Recombinant human PSMF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Proteasome inhibitor subunit 1 isoform 1, PI31

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

31.9 kDa (291aa) confirmed by MALDI-TOF

PSMF1, Proteasome inhibitor subunit 1 isoform 1, PI31, ATGP1352-10 µg, ATGP1352-20 µg, ATGP1352-50 µg, ATGP1352-100 µg, ATGP1352-250 µg, ATGP1352-500 µg, ATGP1352-1 mg, ATGP1352-10, ATGP1352-20, ATGP1352-50, ATGP1352-100, ATGP1352-250, ATGP1352-500, ATGP1352-1

Tanahashi N. et al. (1999) Mol Biol Rep 26: 3-9.; ; Seeger M. et al. (1997) J. Biol. Chem. 272: 8145-8148.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT,0.1mM PMSF

Enzymes & Proteases

NP_006805

Q92530

Human

MAGLEVLFAS AAPAITCRQD ALVCFLHWEV VTHGYCGLGV GDQPGPNDKK SELLPAGWNN NKDLYVLRYE YKDGSRKLLV KAITVESSMI LNVLEYGSQQ VADLTLNLDD YIDAEHLGDF HRTYKNSEEL RSRIVSGIIT PIHEQWEKAN VSSPHREFPP ATAREVDPLR IPPHHPHTSR QPPWCDPLGP FVVGGEDLDP FGPRRGGMIV DPLRSGFPRA LIDPSSGLPN RLPPGAVPPG ARFDPFGPIG TSPPGPNPDH LPPPGYDDMY L