Recombinant humaan PSMG2-eiwit

PSMG2, also known as proteasome assembly chaperone 2, promotes assembly of the 20S proteasome as part of a heterodimer with PSMG1. The PSMG1-PSMG2 heterodimer binds to the PSMA5 and PSMA7 proteasome subunits, promotes assembly of the proteasome alpha subunits into the heteroheptameric alpha ring and prevents alpha ring dimerization. Recombinant human PSMG2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Proteasome assembly chaperone 2, CLAST3, HCCA3, HsT1707, PAC2, TNFSF5IP1

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31.8 kDa (287aa) confirmed by MALDI-TOF

PSMG2, Proteasome assembly chaperone 2, CLAST3, HCCA3, HsT1707, PAC2, TNFSF5IP1, ATGP1211-10 µg, ATGP1211-20 µg, ATGP1211-50 µg, ATGP1211-100 µg, ATGP1211-250 µg, ATGP1211-500 µg, ATGP1211-1 mg, ATGP1211-10, ATGP1211-20, ATGP1211-50, ATGP1211-100, ATGP1211-250, ATGP1211-500, ATGP1211-1

Hirano Y., et al. (2005) Nature. 437:1381-1385; ; Le Tallec B., et al. (2007) Mol. Cell. 27:660-674

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl

Heat Shock Proteins

NP_064617

Q969U7

Human

MFVPCGE SAPDLAGFTL LMPAVSVGNV GQLAMDLIIS TLNMSKIGYF YTDCLVPMVG NNPYATTEGN STELSINAEV YSLPSRKLVA LQLRSIFIKY KSKPFCEKLL SWVKSSGCAR VIVLSSSHSY QRNDLQLRST PFRYLLTPSM QKSVQNKIKS LNWEEMEKSR CIPEIDDSEF CIRIPGGGIT KTLYDESCSK EIQMAVLLKF VSEGDNIPDA LGLVEYLNEW LQILKPLSDD PTVSASRWKI PSSWRLLFGS GLPPALF