Recombinant humaan PSTPIP1-eiwit

PSTPIP1 binds to the cytoplasmic tail of CD2, an effector of T cell activation and adhesion, negatively affecting CD2-triggered T cell activation. The protein appears to be a scaffold protein and a regulator of the actin cytoskeleton. It has also been shown to bind ABL1, PTPN18, WAS, CD2AP, and PTPN12. Mutations in this gene are a cause of PAPA syndrome. Recombinant human PSTPIP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Proline-serine-threonine phosphatase interacting protein 1, CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

50kDa (439aa)

PSTPIP1, Proline-serine-threonine phosphatase interacting protein 1, CD2BP1, CD2BP1L, CD2BP1S, H-PIP, PAPAS, PSTPIP, ATGP2158-10 µg, ATGP2158-20 µg, ATGP2158-50 µg, ATGP2158-100 µg, ATGP2158-250 µg, ATGP2158-500 µg, ATGP2158-1 mg, ATGP2158-10, ATGP2158-20, ATGP2158-50, ATGP2158-100, ATGP2158-250, ATGP2158-500, ATGP2158-1

Shoham N.G., et al. (2003) Proc. Natl. Acad. Sci. u.S.A. 100:13501-13506; ; Wise C.A., et al. (2002) Hum. Mol. Genet. 11:961-969

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Signal Transduction

NP_003969

O43586

Human

MMPQLQF KDAFWCRDFT AHTGYEVLLQ RLLDGRKMCK DMEELLRQRA QAEERYGKEL VQIARKAGGQ TEINSLRASF DSLKQQMENV GSSHIQLALT LREELRSLEE FRERQKEQRK KYEAVMDRVQ KSKLSLYKKA MESKKTYEQK CRDADDAEQA FERISANGHQ KQVEKSQNKA RQCKDSATEA ERVYRQSIAQ LEKVRAEWEQ EHRTTCEAFQ LQEFDRLTIL RNALWVHSNQ LSMQCVKDDE LYEEVRLTLE GCSIDADIDS FIQAKSTGTE PPAPVPYQNY YDREVTPLTS SPGIQPSCGM IKRFSGLLHG SPKTTSLAAS AASTETLTPT PERNEGVYTA IAVQEIQGNP ASPAQEYRAL YDYTAQNPDE LDLSAGDILE VILEGEDGWW TVERNGQRGF VPGSYLEKL