Recombinant humaan PTGR1-eiwit

PTGR1, also known as LTB4DH, belongs to the NADP-dependent oxidoreductase L4BD family. This enzyme catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4. It is highly expressed in the kidney, liver, and intestine but not in leukocytes. Recombinant human PTGR1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Prostaglandin reductase 1, LTB4DH, PGR1, ZADH3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

38.6 kDa (354aa) confirmed by MALDI-TOF

PTGR1, prostaglandin reductase 1, LTB4DH, PGR1, ZADH3, ATGP1635-10 µg, ATGP1635-20 µg, ATGP1635-50 µg, ATGP1635-100 µg, ATGP1635-250 µg, ATGP1635-500 µg, ATGP1635-1 mg, ATGP1635-10, ATGP1635-20, ATGP1635-50, ATGP1635-100, ATGP1635-250, ATGP1635-500, ATGP1635-1

Yokomizo T., et al. (1996) J. Biol. Chem. 271:2844-2850; ; Tai, H.H., et al. (2002) . Prostaglandins Other Lipid Mediat. 68-69: 483-493.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 10% glycerol, 1mM DTT, 200 mM NaCl

Signal Transduction

NP_036344

Q14914

Human

MGSSHHHHHH SSGLVPRGSH MGSEFMVRTK TWTLKKHFVG YPTNSDFELK TAELPPLKNG EVLLEALFLT VDPYMRVAAK RLKEGDTMMG QQVAKVVESK NVALPKGTIV LASPGWTTHS ISDGKDLEKL LTEWPDTIPL SLALGTVGMP GLTAYFGLLE ICGVKGGETV MVNAAAGAVG SVVGQIAKLK GCKVVGAVGS DEKVAYLQKL GFDVVFNYKT VESLEETLKK ASPDGYDCYF DNVGGEFSNT VIGQMKKFGR IAICGAISTY NRTGPLPPGP PPEIVIYQEL RMEAFVVYRW QGDARQKALK DLLKWVLEGK IQYKEYIIEG FENMPAAFMG MLKGDNLGKT IVKA