Recombinant humaan PTH-eiwit

Human parathyroid hormone (hPTH) is an 84 amino acid residue peptide and one of the main regulators in maintenance of calcium homeostasis. It acts primarily on kidney and bone cells stimulating calcium back resorption or calcium mobilization, respectively. The whole mechanism is still under discussion, but low-dose hPTH triggers cyclic AMP-dependent protein kinase in some populations of bone cells bearing PTH receptors, which stimulates the proliferation of osteoblasts. Human parathyroid hormone (amino acids 32-115) was overexpressed in E. coli and purified to apparent homogeneity by using conventional column chromatography techniques

Human PTH Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50 ng/mL.

PTH, PTH1

E.coli

Human

Non-Tagged

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its ability to induce cAMP accumulation in MC3T3/E1 mouse preosteoblast cells. The ED50 range ≤ 50 ng/mL.

9.55 kDa (85aa) confirmed by MALDI-TOF

PTH, PTH, PTH1, ATGP3317-10 µg, ATGP3317-20 µg, ATGP3317-50 µg, ATGP3317-100 µg, ATGP3317-250 µg, ATGP3317-500 µg, ATGP3317-1 mg, ATGP3317-10, ATGP3317-20, ATGP3317-50, ATGP3317-100, ATGP3317-250, ATGP3317-500, ATGP3317-1

Hodsman AB. et al. (2003) J. Clin. Endocrinol. Metab. 88 (11), 5212-20.; ; Wingender E., et al. (1989) J. Biol. Chem. 264 (8),4367-73.

Zhang H, et al. Facile detection of proteins on a solid-phase membrane by direct binding of dextran-based luminol-biotin chemiluminescent polymer. (Talanta. 2009) {https://pubmed.ncbi.nlm.nih.gov/19576433/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 2mM EDTA

Hormones & Cytokines

NP_000306

P01270

Human

MSVSEIQLMH NLGKHLNSME RVEWLRKKLQ DVHNFVALGA PLAPRDAGSQ RPRKKEDNVL VESHEKSLGE ADKADVNVLT KAKSQ