Recombinant humaan PTK9/TWF1-eiwit

TWF1 is a ubiquitous actin-monomer-binding protein that is composed of two ADF-homology domains. It forms a 1:1 complex with ADP-actin-monomers, inhibits nucleotide exchange on actin monomers and prevents assembly of the monomer into filaments. This protein composed of two ADF/cofilin-like (ADF-H) domains connected by a short linker region and followed by a C-terminal tail of 20 residues. The two ADF-H domains are 20% homologous to ADF/cofilin and to each other. Recombinant human TWF1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Twinfilin-1, PTK9

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

31.5 kDa (277aa) confirmed by MALDI-TOF

TWF1, Twinfilin-1, PTK9, ATGP1322-10 µg, ATGP1322-20 µg, ATGP1322-50 µg, ATGP1322-100 µg, ATGP1322-250 µg, ATGP1322-500 µg, ATGP1322-1 mg, ATGP1322-10, ATGP1322-20, ATGP1322-50, ATGP1322-100, ATGP1322-250, ATGP1322-500, ATGP1322-1

Beeler J.F., et al. (1994) Mol. Cell. Biol. 14:982-988; ; Yu L.-R., et al. (2007) J. Proteome Res. 6:4150-4162

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.15M NaCl, 1mM DTT

Cytoskeleton

AAH22344

Q12792

Human

MGSSHHHHHH SSGLVPRGSH MGSHMMLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD