Recombinant humaan PTRH2-eiwit

PTRH2 (peptidyl-tRNA hydrolase 2), also known as BIT1, is a mitochondrial protein. During apoptosis, PTRH2 is released from the mitochondria to the cytoplasm. Once in the cytoplasm, PTRH2 regulates the function of two transcriptional regulators, TLE5 and TLE1, thereby promoting caspase-independent cell death. The natural substrate for PTRH2 may be petidyl-tRNAs which drop off the ribosome during protein synthesis. Recombinant human PTRH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Peptidyl-tRNA hydrolase 2, BIT1, PTH2, CGI-147, Bcl-2 inhibitor of transcription 1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

14.9 kDa (137aa) confirmed by MALDI-TOF

PTRH2, Peptidyl-tRNA hydrolase 2, BIT1, PTH2, CGI-147, Bcl-2 inhibitor of transcription 1, ATGP0830-10 µg, ATGP0830-20 µg, ATGP0830-50 µg, ATGP0830-100 µg, ATGP0830-250 µg, ATGP0830-500 µg, ATGP0830-1 mg, ATGP0830-10, ATGP0830-20, ATGP0830-50, ATGP0830-100, ATGP0830-250, ATGP0830-500, ATGP0830-1

Jan Y., et al. (2004) Cell. 116 (5) :751-62.; ; De Pereda JM., et al. (2004) J Biol Chem. 279 (9) :8111-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Apoptosis

NP_057161

Q9Y3E5

Human

MGSSHHHHHH SSGLVPRGSH MEYKMILVVR NDLKMGKGKV AAQCSHAAVS AYKQIQRRNP EMLKQWEYCG QPKVVVKAPD EETLIALLAH AKMLGLTVSL IQDAGRTQIA PGSQTVLGIG PGPADLIDKV TGHLKLY