Recombinant humaan PTS-eiwit

6-Pyruvoyltetrahydropterin synthase (PTS), also known as PTPS, belongs to the family of lyases, specifically those carbon-oxygen lyases acting on phosphates. The enzyme encoded by this gene catalyzes the elimination of inorganic triphosphate from dihydroneopterin triphosphate, which is the second and irreversible step in the biosynthesis of tetrahydrobiopterin from GTP. Tetrahydrobiopterin, also known as BH (4), is an essential cofactor and regulator of various enzyme activities, including enzymes involved in serotonin biosynthesis and NO synthase activity. Mutations in this gene result in hyperphenylalaninemia. Recombinant human PTS, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

6-pyruvoyltetrahydropterin synthase, PTPS, PTP synthase, 6-pyruvoyltetrahydropterin synthase 6 pyruvoyl tetrahydrobiopterin synthase, 6 pyruvoyl tetrahydropterin synthase, 6 pyruvoyltetrahydropterin synthase, EC 4.2.3.12, FLJ97081

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

18.5 kDa (165aa) confirmed by MALDI-TOF

PTS, 6-pyruvoyltetrahydropterin synthase, PTPS, PTP synthase, 6-pyruvoyltetrahydropterin synthase 6 pyruvoyl tetrahydrobiopterin synthase, 6 pyruvoyl tetrahydropterin synthase, 6 pyruvoyltetrahydropterin synthase, EC 4.2.3.12, FLJ97081, ATGP0452-10 µg, ATGP0452-20 µg, ATGP0452-50 µg, ATGP0452-100 µg, ATGP0452-250 µg, ATGP0452-500 µg, ATGP0452-1 mg, ATGP0452-10, ATGP0452-20, ATGP0452-50, ATGP0452-100, ATGP0452-250, ATGP0452-500, ATGP0452-1

Thony B., et al. (1992) Biochem Biophys Res Commun. 189:1437-43. ; ; Milstien S., et al. (1989) J Biol Chem. 264 (14) :8066-73.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Signal Transduction

NP_000308

Q03393

Human

MSTEGGGRRC QAQVSRRISF SASHRLYSKF LSDEENLKLF GKCNNPNGHG HNYKVVVTVH GEIDPATGMV MNLADLKKYM EEAIMQPLDH KNLDMDVPYF ADVVSTTENV AVYIWDNLQK VLPVGVLYKV KVYETDNNIV VYKGE