Recombinant humaan PTTG1-eiwit

PTTG1, also known as Securin, is primarily involved in the regulation of sister chromatid separation during cell division. It has two identified roles; the first one is to help the transport of separase (cysteine protease) to the nucleus and the second role is to inhibit the catalytic activity of separase. It is ubiquitinated by the Anaphase Promoting Complex (APC), and then degraded by the Proteasome, releasing separase. Recombinant PTTG1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Pituitary tumor-transforming protein 1, EAP1, HPTTG, PTTG, TuTR1, SECuRIN, Pituitary tumor-transforming protein 1 ESP1 ASSOCIATED PROTEIN 1, Esp1-associated protein, MGC126883, MGC138276, Pds1, PTTG1 protein, PTTG1, Pituitary tumor transforming 1, PTTG 1, EAP 1, Cut2, Pituitary tumor transforming protein 1, AW555095, Pituitary tumor-transforming 1, isoform CRA_a, Pituitary tumor-transforming 1, isoform CRA_b, hPTTG, Pituitary tumor-transforming gene 1, TuTR 1, Pttg3, Pituitary tumor-transforming gene 1 protein, C87862, TuMOR TRANSFORMING 1, Tumor transforming protein 1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

24.1 kDa (222aa) confirmed by MALDI-TOF

PTTG1, Pituitary tumor-transforming protein 1, EAP1, HPTTG, PTTG, TuTR1, SECuRIN, Pituitary tumor-transforming protein 1 ESP1 ASSOCIATED PROTEIN 1, Esp1-associated protein, MGC126883, MGC138276, Pds1, PTTG1 protein, PTTG1, Pituitary tumor transforming 1, PTTG 1, EAP 1, Cut2, Pituitary tumor transforming protein 1, AW555095, Pituitary tumor-transforming 1, isoform CRA_a, Pituitary tumor-transforming 1, isoform CRA_b, hPTTG, Pituitary tumor-transforming gene 1, TuTR 1, Pttg3, Pituitary tumor-transforming gene 1 protein, C87862, TuMOR TRANSFORMING 1, Tumor transforming protein 1, ATGP0366-10 µg, ATGP0366-20 µg, ATGP0366-50 µg, ATGP0366-100 µg, ATGP0366-250 µg, ATGP0366-500 µg, ATGP0366-1 mg, ATGP0366-10, ATGP0366-20, ATGP0366-50, ATGP0366-100, ATGP0366-250, ATGP0366-500, ATGP0366-1

Chien W., et al. (2000) J Biol Chem. 275 (25) :19422-7.; ; Tariq Hamid., et al. (2005) Molecular Cancer. 4 (1) :3.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol, 1mM EDTA, 0.1mM PMSF

Cancer

NP_004210

O95997

Human

MGSSHHHHHH SSGLVPRGSH MATLIYVDKE NGEPGTRVVA KDGLKLGSGP SIKALDGRSQ VSTPRFGKTF DAPPALPKAT RKALGTVNRA TEKSVKTKGP LKQKQPSFSA KKMTEKTVKA KSSVPASDDA YPEIEKFFPF NPLDFESFDL PEEHQIAHLP LSGVPLMILD EERELEKLFQ LGPPSPVKMP SPPWESNLLQ SPSSILSTLD VELPPVCCDI DI