Recombinant humaan PTZK1-eiwit

PDZK1 is a PDZ domain-containing scaffolding protein. This protein mediates the localization of cell surface proteins and plays a critical role in cholesterol metabolism by regulating the HDL receptor, scavenger receptor class B type 1. Single nucleotide polymorphisms in this gene may be associated with metabolic syndrome, and overexpression of this gene may play a role in drug resistance of multiple myeloma. Recombinant human PDZK1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Na (+) /H (+) exchange regulatory cofactor NHE-RF3, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

59 kDa (542aa)

PDZK1, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, Na (+) /H (+) exchange regulatory cofactor NHE-RF3, CAP70, CLAMP, NHERF-3, NHERF3, PDZD1, ATGP2075-10 µg, ATGP2075-20 µg, ATGP2075-50 µg, ATGP2075-100 µg, ATGP2075-250 µg, ATGP2075-500 µg, ATGP2075-1 mg, ATGP2075-10, ATGP2075-20, ATGP2075-50, ATGP2075-100, ATGP2075-250, ATGP2075-500, ATGP2075-1

Kocher O, Yesilaltay A, et al. (2003) . J Biol Chem. 278 (52) :52820-5.; ; Inoue J, Otsuki T, et al. (2004) . Am J Pathol. 165 (1) :71-81.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_001188254

Q5T2W1

Human

MGSSHHHHHH SSGLVPRGSH MGSMTSTFNP RECKLSKQEG QNYGFFLRIE KDTEGHLVRV VEKCSPAEKA GLQDGDRVLR INGVFVDKEE HMQVVDLVRK SGNSVTLLVL DGDSYEKAVK TRVDLKELGQ SQKEQGLSDN ILSPVMNGGV QTWTQPRLCY LVKEGGSYGF SLKTVQGKKG VYMTDITPQG VAMRAGVLAD DHLIEVNGEN VEDASHEEVV EKVKKSGSRV MFLLVDKETD KRHVEQKIQF KRETASLKLL PHQPRIVEMK KGSNGYGFYL RAGSEQKGQI IKDIDSGSPA EEAGLKNNDL VVAVNGESVE TLDHDSVVEM IRKGGDQTSL LVVDKETDNM YRLAHFSPFL YYQSQELPNG SVKEAPAPTP TSLEVSSPPD TTEEVDHKPK LCRLAKGENG YGFHLNAIRG LPGSFIKEVQ KGGPADLAGL EDEDVIIEVN GVNVLDEPYE KVVDRIQSSG KNVTLLVCGK KAYDYFQAKK IPIVSSLADP LDTPPDSKEG IVVESNHDSH MAKERAHSTA SHSSSNSEDT EM