Recombinant humaan PURB-eiwit

PuRB is a sequence-specific, single-stranded DNA-binding protein. It binds preferentially to the single strand of the purine-rich element termed PuR, which is present at origins of replication and in gene flanking regions in a variety of eukaryotes from yeasts through humans. Thus, it is implicated in the control of both DNA replication and transcription. Deletion of this gene has been associated with myelodysplastic syndrome and acute myelogenous leukemia. Recombinant human PuRB protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Transcriptional activator protein Pur-beta, Purine-rich element binding protein B, PuRBETA

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.6 kDa (335aa) confirmed by MALDI-TOF

PuRB, Transcriptional activator protein Pur-beta, Purine-rich element binding protein B, PuRBETA, ATGP2686-10 µg, ATGP2686-20 µg, ATGP2686-50 µg, ATGP2686-100 µg, ATGP2686-250 µg, ATGP2686-500 µg, ATGP2686-1 mg, ATGP2686-10, ATGP2686-20, ATGP2686-50, ATGP2686-100, ATGP2686-250, ATGP2686-500, ATGP2686-1

Bergemann A.D., Ma Z.-W., et al. (1992) Mol. Cell. Biol. 12:5673-5682

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_150093

Q96QR8

Human

MGSSHHHHHH SSGLVPRGSH MGSMADGDSG SERGGGGGPC GFQPASRGGG EQETQELASK RLDIQNKRFY LDVKQNAKGR FLKIAEVGAG GSKSRLTLSM AVAAEFRDSL GDFIEHYAQL GPSSPEQLAA GAEEGGGPRR ALKSEFLVRE NRKYYLDLKE NQRGRFLRIR QTVNRGGGGF GAGPGPGGLQ SGQTIALPAQ GLIEFRDALA KLIDDYGGED DELAGGPGGG AGGPGGGLYG ELPEGTSITV DSKRFFFDVG CNKYGVFLRV SEVKPSYRNA ITVPFKAWGK FGGAFCRYAD EMKEIQERQR DKLYERRGGG SGGGEESEGE EVDED