Recombinant humaan Purinenucleoside fosforylase/PNP-eiwit

PNP belongs to the PNP/MTAP phosphorylase family of proteins. This protein catalyzes the reversible phosphorolysis of ribonucleosides and 2'-deoxyribonucleosides with specificity for guanine, hypoxanthine and their analogs. PNP deficiency is a rare autosomal recessive genetic disease associated with a severe defect in T-lymphocyte function and neurologic disorder in children, comprising four percent of combined immunodeficiency cases. Recombinant human PNP protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Specific activity is > 120 units/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

Purine nucleoside phosphorylase, NP, Inosine phosphorylase, PRO1837, PuNP

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 120unit/mg, and is defined as the amount of enzyme that phosphorolysis of 1.0 umole of inosine with inorganic phosphate per minute at pH 7.5 at 25C.

34.2 kDa (309aa) confirmed by MALDI-TOF

Purine nucleoside phosphorylase, PNP, ATGP0786-10 µg, ATGP0786-20 µg, ATGP0786-50 µg, ATGP0786-100 µg, ATGP0786-250 µg, ATGP0786-500 µg, ATGP0786-1 mg, ATGP0786-10, ATGP0786-20, ATGP0786-50, ATGP0786-100, ATGP0786-250, ATGP0786-500, ATGP0786-1

Dautant A., et al. (2010) J Biol Chem. 285 (38) :29502-10.; ; Balakrishnan K., et al. (2010) Blood. 116 (6) :886-92.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 2mM DTT, 10% glycerol

Neuroscience

NP_000261

P00491

Human

MENGYTYEDY KNTAEWLLSH TKHRPQVAII CGSGLGGLTD KLTQAQIFDY GEIPNFPRST VPGHAGRLVF GFLNGRACVM MQGRFHMYEG YPLWKVTFPV RVFHLLGVDT LVVTNAAGGL NPKFEVGDIM LIRDHINLPG FSGQNPLRGP NDERFGDRFP AMSDAYDRTM RQRALSTWKQ MGEQRELQEG TYVMVAGPSF ETVAECRVLQ KLGADAVGMS TVPEVIVARH CGLRVFGFSL ITNKVIMDYE SLEKANHEEV LAAGKQAAQK LEQFVSILMA SIPLPDKAS