Recombinant humaan PYCR2-eiwit

PYCR2 also known as pyrroline-5-carboxylate reductase 2 isoform 1, belongs to the pyrroline-5-carboxylate reductase family. This protein catalyzes the conversion of pyrroline-5-carboxylate to proline, which is the last step in proline biosynthesis. The 3 substrates of this enzyme are L-proline, NAD+, and NADP+, whereas its 4 products are 1-pyrroline-5-carboxylate, NADH, NADPH, and H+. Recombinant human PYCR2, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36 kDa (343aa) confirmed by MALDI-TOF

PYCR2, Pyrroline-5-carboxylate reductase 2 isoform 1, P5CR2, ATGP3066-10 µg, ATGP3066-20 µg, ATGP3066-50 µg, ATGP3066-100 µg, ATGP3066-250 µg, ATGP3066-500 µg, ATGP3066-1 mg, ATGP3066-10, ATGP3066-20, ATGP3066-50, ATGP3066-100, ATGP3066-250, ATGP3066-500, ATGP3066-1

De Ingeniis J, et al. (2012) . PLoS One. 7 (9) :e45190.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 50% glycerol, 5mM DTT, 1mM EDTA

Metabolism

NP_037460

Q96C36

Human

MSVGFIG AGQLAYALAR GFTAAGILSA HKIIASSPEM NLPTVSALRK MGVNLTRSNK ETVKHSDVLF LAVKPHIIPF ILDEIGADVQ ARHIVVSCAA GVTISSVEKK LMAFQPAPKV IRCMTNTPVV VQEGATVYAT GTHALVEDGQ LLEQLMSSVG FCTEVEEDLI DAVTGLSGSG PAYAFMALDA LADGGVKMGL PRRLAIQLGA QALLGAAKML LDSEQHPCQL KDNVCSPGGA TIHALHFLES GGFRSLLINA VEASCIRTRE LQSMADQEKI SPAALKKTLL DRVKLESPTV STLTPSSPGK LLTRSLALGG KKD