Recombinant humaan PYCRL/PYCR3-eiwit

PYCRL belongs to the pyrroline-5-carboxylate reductase family and functions as a homodecamer. It may play a critical role in proline bio-synthesis. Proline functions as a non-enzymatic antioxidant to minimize damage caused by reactive oxygen species (ROS) in microorganisms, animals and plants. In the last step of proline biosynthesis, PYCRL catalyzes the reduction of aldehyde dehydrogenase 4A1 (ALDH4A1) to proline using NAD (P) H as the cofactor. Recombinant human PYCRL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Pyrroline-5-carboxylate reductase 3, P5C reductase 3, P5CR 3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

31 kDa (297aa) confirmed by MALDI-TOF

P5C reductase 3, P5C reductase3, P5CR 3, P5CR3, PYCRL, Pyrroline 5 carboxylate reductase 3, Pyrroline-5-carboxylate reductase 3, ATGP1906-10 µg, ATGP1906-20 µg, ATGP1906-50 µg, ATGP1906-100 µg, ATGP1906-250 µg, ATGP1906-500 µg, ATGP1906-1 mg, ATGP1906-010, ATGP1906-020, ATGP1906-050, ATGP1906-100, ATGP1906-250, ATGP1906-500, ATGP1906-01M

Nocek B., et al. (2005) J Mol Biol. 354:91-106.; ; Krishnan N., et al. (2008) Free Radic Biol Med. 44: 671-681.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Metabolism

AAH07993.1

Q53H96

Human

MAAAEPS PRRVGFVGAG RMAGAIAQGL IRAGKVEAQH ILASAPTDRN LCHFQALGCR TTHSNQEVLQ SCLLVIFATK PHVLPAVLAE VAPVVTTEHI LVSVAAGVSL STLEELLPPN TRVLRVLPNL PCVVQEGAIV MARGRHVGSS ETNLLQHLLE ACGRCEEVPE AYVDIHTGLS GSGVAFVCAF SEALAEGAVK MGMPSSLAHR IAAQTLLGTA KMLLHEGQHP AQLRSDVCTP GGTTIYGLHA LEQGGLRAAT MSAVEAATCR AKELSRK